PCR Supplies
Sigma Aldrich Fine Chemicals Biosciences Taq DNA Polymerase dNTPack
Taq DNA Polymerase is a highly processive 5-3 DNA polymerase that lacks 3-5 exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa.Taq DNA Polymerase was originally isolated from the thermophilic eubacterium Thermus aquaticus BM a strain lacking Taq I restriction endonuclease. The enzyme preparation obtained from E.coli is free of nonspecific engo- or exonucleases according to the current quality control procedures.
Sigma Aldrich Fine Chemicals Biosciences ReadyScriptR cDNA Synthesi
ReadyScript cDNA Synthesis Mix is a sensitive and easy-to-use SOLUTIONon for two-step RT-PCR. This 5X concentrated mix provides all necessary components (except RNA template) for first-strand synthesis including buffer dNTPs MgCl2 primers RNase inhibitor protein ReadyScript reverse transcriptase and stabilizers. ReadyScript is a RNase H() derivative of MMLV reverse transcriptase optimized for reliable cDNA synthesis over a wide dynamic range of input RNA. The unique blend of oligo (dT) and random primers in the ReadyScript cDNA Synthesis Mix works exceptionally well with a wide variety of targets. It is optimized for the production of targets 1kb in length. ReadyScript cDNA Synthesis Mix produces excellent results in both real-time and conventional RT-PCR.
Active Motif Human Positive Control Primer Set MYT1
Human Control qPCR Primer Sets were designed to serve as positive or negative ChIP controls when performing chromatin immunoprecipitation with human samples. Each primer set is a mixture of forward and reverse primers that have been validated for qPCR and endpoint PCR of ChIP samples from multiple human cell lines.
Sigma Aldrich Fine Chemicals Biosciences fastStartTM Taq DNA Polyme
The enzyme dNTPack comprises fastStart Taq DNA Polymerase and a ready-to-use SOLUTIONon of PCR grade nucleotides. fastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified thermostable recombinant Taq DNA polymerase is inactive at temperatures below 75 C but is activated by a 2 to 4 minute heat activation step at 95 C. Since it is inactive at low temperatures fastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH SOLUTIONon to enable the enzyme to handle a wide range of templates are also supplied.
Sigma Aldrich Fine Chemicals Biosciences fastStart Universal SYBR G
fastStart(TM) Universal SYBR(R) Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX. SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye which is included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence. Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(TM) Taq DNA Polymerase make the modified enzyme inactive at room temperature (15 to 25C). Therefore there is no elongation during the period when primers can non-specifically bind. fastStart(TM) Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e. a pre-incubation step at 95C).
Sigma Aldrich Fine Chemicals Biosciences fastStartTM Taq DNA Polyme
fastStart(TM) Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified thermostable recombinant Taq DNA polymerase is inactive at temperatures below 75 C but is activated by a 2 to 4 minute heat activation step at 95 C. Since it is inactive at low temperatures fastStart(TM) Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. fastStart(TM) Taq DNA Polymerase is an ideal tool for hot start PCR because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
Sigma Aldrich Fine Chemicals Biosciences KOD OneTM PCR Master Mix
KOD One(TM) PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator enabling fast PCR with an extension time of 5 sec/ kb for template DNA 10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5-3 polymerase activity and 3-5 exonuclease activity. Upon heat-activation the 3-5 proof-reading ability of the enzyme generates blunt-end PCR products.
Alkali Scientific Radiant™ SYBR Green Fluorescein qPCR Kit, 500 Reactions, (5 x 1mL)
Radiant™ SYBR Green Flourescein qPCR Kit: Next-generation PCR buffer formulations for maximum PCR efficiency and reaction speed. Novel hot-start chemistry for improved specificity and sensitivity. Broad range detection for increased reliability in low-copy assays Versatile 2X mixes, suitable for both ultra-fast and standard cycling methods Compatible with BioRad iCycler™, MyIQ™ & IQ5™
New England Biolabs, Inc. FauI – 200 units
A restriction endonuclease that recognizes the sequence CCCGCNNNN^NN_.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Standard Buffer, Magnesium free and Triton free, 500U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq is Triton/magnesium free 10x Standard Buffer.
As One International Inc Taq DNA Polymerase 5 U/ul 10x Combination Buffer, 1,000U
Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa. Taq DNA Polymerase has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity. The enzyme lacks a 3' to 5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A prime overhang, which makes the enzyme ideal for TA cloning. Included with Taq is 10x Combination Buffer.
New England Biolabs, Inc. Taq DNA Polymerase with Standard Taq (Mg-free) Buffer – 2000 units
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5' to 3' polymerase activity and a 5' flap endonuclease activity. It is supplied with 10X Standard Taq (Mg-free) Reaction Buffer and MgCl2. 10X Standard Taq (Mg-free) Reaction Buffer is detergent-free and designed to be compatible with existing assay systems.
Qiagen Beverly Inc PHI29 DNA POLYMERASE DNAHIGH
DNA Polymerase, Phi-29 DNA Polymerase High Conc.; Conc. 100,000 U/ul; 2,000 U; incl. 10X buffer
